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移动式叶绿素荧光成像系统

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产品描述

 

移动式叶绿素荧光成像系统PlantExplorerXS是由慧诺瑞德和荷兰PhenoVation公司联合推出的专门针对大田、温室、气候室和实验室场景的可以移动的叶绿素荧光测量系统。配备移动式升降平台车、内置电脑的叶绿素荧光成像单元、移动电源、显示单元和操作单元。叶绿素荧光成像单元可以升降和旋转,既可以测量不同高度的植物冠层,也可以倾斜或水平角度测量穗(麦穗、稻穗、谷穗等)、荚果(大豆、油菜等)、果实(番茄、黄瓜、葡萄、柑橘等)、叶片或冠层。

 

该系统成像面积为18x18cm,具备500万像素高清成像,同时具备“调制”和“非调制”叶绿素荧光成像测量功能,既可以测量光合生理,也可以测量形态结构,同时配备功能强大的控制和分析软件,且可以对大量数据进行批处理分析。该系统性价比超高,无论室内还是大田,都是进行植物表型、光合生理、植物抗逆、植物病理、育种、功能基因组、突变株筛选、种子生理/病理等研究的必备利器。
 

 

功能特性

  • 大田、温室、气候室、实验室进行移动式测量
  • 叶绿素荧光成像单元可以升降、旋转
  • 叶绿素荧光成像和表型分析同步测量
  • 同时具备调制和非调制叶绿素荧光测量功能
  • 出色的高清相机(500万像素)、高信噪比成像
  • 16位图像格式,无与伦比的成像质量
  • 光源、相机、滤光片、电脑一体化设计
  • 无可见镜头畸变,无需图像校正
  • 成像范围18 x 18cm
  • 多种测量protocol可选,允许用户编辑设定自己的protocol,包括但不限于Fv/Fm测量、标准诱导曲线测量、暗弛豫测量、OJIP快速诱导动力学测量等等。
  • 可进行功能强大的延时成像测量
  • 自动计算荧光参数和表型参数
  • 具备图像数据批处理分析功能
  • 提供多种功能强大的图像分割功能
  • 对所有图像数据均提供数据分级(用户自定义范围)并进行图像化显示,并允许对分级筛选后的数据叠加到可见光图像上展示
  • 图像背景、伪彩色标尺均有多种选择
  • 允许用户自定义多种ROI(性状、数目、分布等)并对ROI的数据自动分析
  • 嵌入式电脑进行精确的成像、时间控制、光强控制和数据存储
  • 功能强大的控制和分析软件
  • 特别适合突变株筛选、育种材料/组合筛选、抗逆研究、病理研究、种子研究、果实研究、功能基因组学等

主要技术参数

  • 基本组成:移动式升降平台、叶绿素荧光成像单元、移动电源、显示单元、操作单元等
  • 叶绿素荧光成像方式:“调制”测量 +“费调制”测量
  • 调制测量光:蓝色LED, 450nm,半峰全宽20nm,最大光强4000 umol m-2 s-1 ,独立触发
  • Kautsky测量光:蓝色LED, 450nm,半峰全宽20nm,最大光强4000 umol m-2 s-1
  • 饱和脉冲:蓝色LED, 450nm,半峰全宽20nm,最大光强4000 umol m-2 s-1,独立触发
  • 时间分辨动力学光化光:红光LED,660nm,最大光强800 umol m-2 s-1
  • 远红光:LED,735nm,半峰全宽20nm,35W
  • 相机:CMOS传感器,500万像素
  • 颜色深度:12bit
  • 标准帧率:37.5 FPS
  • 图像格式:16bit
  • 相机光谱范围:400~1000 nm
  • 接口:3个USB3.0,1个以太网口,1个HDMI接口
  • 嵌入式电脑:4核处理器,8G内存,256G固态硬盘
  • 成像面积:18cm x 18cm
  • 升降高度:0-1200mm(高度可定制)
  • 旋转角度:-90° ~ 90°
  • 显示单元:15.6寸触摸显示屏
  • 供电:35万mAh移动电源,额定容量1260Wh,峰值功耗1000W,待机功耗35W
  • 系统尺寸:600mm x 720mm x 2000mm(长x宽x高)

 

 

测量参数

  • 调制叶绿素荧光参数:Fo、Fm、Fv/Fm、dFq/Fm=DF/Fm、Fs’、Fm’、Fo’、Fq’/Fm’=Fv’/Fm’、rETR、NPQ、Y(NO)、Y(NPQ)、qN、qP、qL、1-qP和1-qL等;
  • 非调制叶绿素荧光参数:Fo、Fi、Fm、1-Fi/Fm、IC-Area、IC-Area/Fv、PI、Rfd、dRfd、RfdFm和RfdFt等;
  • 表型参数:(植物、种子、果实的)数目、轮廓面积、长度、宽度、凸包点数、凸包面积、凸包面积/轮廓面积、最小外接圆(质心、半径、面积)、最小外接矩形(长、宽、面积、角度、alpha)和骨架等。

 

 

 

 

 

利用PhenoVation叶绿素荧光成像技术发表的部分文献

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  3. Farooq M, van Dijk A D J, Nijveen H, et al. (2021) Prior Biological Knowledge Improves Genomic Prediction of Growth-Related Traits in Arabidopsis thaliana. Frontiers in Genetics, 11:609117. doi: 10.3389/fgene.2020.609117
  4. He Y, Li Y, Yao Y et al. (2021) Overexpression of watermelon m6A methyltransferase ClMTB enhances drought tolerance in tobacco by mitigating oxidative stress and photosynthesis inhibition and modulating stress-responsive gene expression. Plant Physiology and Biochemistry, 168: 340-352.
  5. Wang W, Liu D, Qin M et al. (2021) Effects of Supplemental Lighting on Potassium Transport and Fruit Coloring of Tomatoes Grown in Hydroponics. International Journal of Molecular Sciences, 22(5): 2687 https://doi.org/10.3390/ijms22052687
  6. Singh R R, Pajar J A, Audenaert K, et al. (2021) Induced Resistance by Ascorbate Oxidation Involves Potentiating of the Phenylpropanoid Pathway and Improved Rice Tolerance to Parasitic Nematodes. Frontiers in Plant Science, 12:713870. doi: 10.3389/fpls.2021.713870
  7. Vidak M, Lazarevic B, Petek M, et al. (2021) Multispectral Assessment of Sweet Pepper (Capsicum annuum L.) Fruit Quality Affected by Calcite Nanoparticles. Biomolecules, 11(6), 832; https://doi.org/10.3390/biom11060832
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  10. Meng L, Mestdagh H, Ameye M, et al. (2021) Phenotypic variation of Botrytis cinerea Isolates is influenced by spectral light quality. Frontiers in Plant Science, 11:1233. doi: 10.3389/fpls.2020.01233
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  13. Tan J, de Zutter N, de Saeger S, et al. (2021) Presence of the Weakly Pathogenic Fusarium poae in the Fusarium Head Blight Disease Complex Hampers Biocontrol and Chemical Control of the Virulent Fusarium graminearum Pathogen. Frontiers in Plant Science, https://doi.org/10.3389/fpls.2021.641890
  14. Flood P, Theeuwen T, Schneeberger K, Keizer P, Kruijer W, et al. (2020) Reciprocal cybrids reveal how organellar genomes affect plant phenotypes. Nature Plants, 10.1038/s41477-019-0575-9ff. ffhal-02392124v2f
  15. Velivelli S L S, Czymmek K J, Li H, Shaw J B, Buchko G W, Shah D M. (2020) Antifungal symbiotic peptide NCR044 exhibits unique structure and multifaceted mechanisms of action that confer plant protection. PNAS, DOI: 10.1073/pnas.2003526117
  16. Bhatnagar N, Pandey S. (2020) Heterotrimeric G-Protein Interactions Are Conserved Despite Regulatory Element Loss in Some Plants. Plant Physiology, DOI: https://doi.org/10.1104/pp.20.01309
  17. Venneman J, Vandermeersch L, Walgraeve C et al. (2020) Respiratory CO2 Combined With a Blend of Volatiles Emitted by Endophytic Serendipita Strains Strongly Stimulate Growth of Arabidopsis Implicating Auxin and Cytokinin Signaling. Frontiers in Plant Science, https://doi.org/10.3389/fpls.2020.544435
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  20. Zhang H, Chen Y, Niu Y, Zhang X, Zhao J, Sun L, Wang H, Xiao J, Wang X. (2020) Characterization and fine mapping of a leaf yellowing mutant in common wheat. Plant Growth Regulation, https://doi.org/10.1007/s10725-020-00633-0
  21. Jin X, Zarco-Tejada P, Schmidhalter U, Reynolds M P et al. (2020) High-throughput estimation of crop traits: A review of ground and aerial phenotyping platforms. IEEE Geoscience and Remote Sensing Magazine, DOI: 10.1109/MGRS.2020.2998816
  22. Sheng X-G, Branca F, Zhao Z-Q et al. (2020) Identification of Black Rot Resistance in a Wild Brassica Species and Its Potential Transferability to Cauliflower. Argonomy, 10: 1400. doi:10.3390/agronomy10091400
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关键词:
叶绿素荧光
叶绿素荧光成像
光合作用
光合表型
植物表型
作物表型
表型组学
生理表型
PlantExplorer
PhenoVation
PhenoTrait
慧诺瑞德
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